Efficacy of ozonized Olive oil in the treatment of tinea pedis.

Menendez S , Falcon L , Simon DR , Landa N .

Centro de Investigaciones del Ozono, Apartado Postal 6880, Playa, Cuba. ozono@infomed.sld.cu

Ozonized Olive oil has a remarkable gerrmicidal action. In the present study, the efficacy of Ozonated Olive Oil in the treatment of tinea pedis was demonstrated in a controlled randomized phase III assay, comparing topical Ozonated Olive Oil with ketoconazole cream 2% (Nizoral) in 200 patients (100 in each group). The treatment administered was twice per day for a period of 6 weeks. The efficacy was evaluated clinically (disappearance of all lesions, with or without negative mycological results) and mycologically (negative culture results). A complete clinical and mycological cure was obtained in 75 and 81% for Ozonated Olive Oil and ketoconazole, respectively, with no significant differences between both groups. No side-effects or bacterial super-infections were observed. Patients were evaluated 6 months after the end of the treatment and no recurrence was observed in the Ozonated Olive Oil group. Ozonated Olive Oil can be an effective alternative low-cost antimycotic drug.

Publication Types: Clinical Trial , Clinical Trial, Phase III , Randomized Controlled Trial
PMID: 12572723 [PubMed - indexed for MEDLINE]
       The dual action of ozone on the ski
Valacchi G , Fortino V , Bocci V .

Department of Physiology, University of Siena, Siena 53100, Italy. gvalacchi@ucdavis.edu

The aim of this brief review is to summarize the recent literature on the effect of ozone (O3) on cutaneous tissues. Recently it has been reported that a chronic contact with O3 can be deleterious for the skin. Our group and others have shown a progressive depletion of antioxidant content in the stratum corneum and this can then lead to a cascade of effects resulting in an active cellular response in the deeper layers of the skin. Using an in vivo model we have shown an increase of proliferative, adaptive and proinflammatory cutaneous tissue responses. On the other hand the well known activity of O3 as a potent disinfectant and oxygen (O2) donor has been also studied for therapeutic use. Two approaches have been denoibed. The first consists of a quasi-total body exposure in a thermostatically controlled cabin. This treatment has proved to be useful in patients with chronic limb ischaemia. The second approach is based on the topical application of ozonated olive oil in several kinds of skin infection (from soreness to diabetic ulcers, burns, traumatic and surgical wounds, abscesses and skin reactions after radiotherapy). We and other authors have observed a striking cleansing effect with improved oxygenation and enhanced healing of these conditions. It is now clear that, on the skin, O3, like other drugs, poisons and radiation, can display either a damaging effect from a long exposure or a beneficial effect after a brief exposure to O2 and O3 or to the application of ozonated oil to chronic wounds.

PMID: 16307642 [PubMed - in process]
       Effects of ozone on how well split-thickness skin grafts took in war wounds. Results of prospective study.

Turcic J , Hancevic J , Antoljak T , Zic R , Alfirevic I .

Zagreb University School of Medicine, Department of Surgery, Clinical Hospital Center Rebro, Croatia.

In a prospective study on 35 wounded persons we examined the effects of ozone on how well split-thickness skin grafts took in war wounds. Each of the 35 wounded persons hat at least two similar gunshot wounds, one on the lower leg or forearm and the other on the upper leg or upper arm. During the first 10 days all wounds were treated with 10% NaCl water solution dressings until the moment when healthy granulations were observed. Thereafter, the defects were covered with split-thickness skin grafts according to Thiersch. For technical reasons we treated grafts on the lower leg and forearm with ozone following the usual scheme. Grafts on the upper leg or upper arm were treated in the conventional way and they served as a control group. The results obtained in the group followed up are presented by percentage of graft takes after 10 days and accordingly compared with the results obtained in the control group. There was a higher percentage of takes in ozone-treated split-thickness skin grafts. More than 74.3% of the split-thickness skin grafts treated with ozone had a take of more than 75% of the covered surface as apposed to only 40% of the grafts treated with the conventional method. The results in these two groups were compared with a chi square matched pair test. Difference in take of the skin grafts in these two groups was statistically significant at P < 0.01.

Publication Types: Clinical Trial , Randomized Controlled Trial
PMID: 7791484 [PubMed - indexed for MEDLINE]
 
      Therapeutic efficacy of ozone in patients with diabetic foot  Gregorio Martínez-Sáncheza, Saied M. Al-Dalaina, Silvia Menéndezb, Lamberto Rec, Attilia Giulianid, Eduardo Candelario-Jalila, Hector Álvareze, José Ignacio Fernández-Montequíne and Olga Sonia Leóna, ,
a Center of Studies for Research and Biological Evaluation (CEIEB-IFAL), University of Havana, Havana 10400, Cuba
bOzone Research Center, Cuba
cLaboratory of Pharmacological Biotechnology, University of Ancona, 60131 Ancona, Italy
dDepartment of Chemistry and Medical Biochemistry, University of Milan, Via Saldini, 50-20133 Milan, Italy
eInstitute of Angiology and Vascular Surgery, Cuba
accepted 1 August 2005.  Available online 29 September 2005.

  Abstract Oxidative stress is suggested to have an important role in the development of complications in diabetes. Because ozone therapy can activate the antioxidant system, influencing the level of glycemia and some markers of endothelial cell damage, the aim of this study was to investigate the therapeutic efficacy of ozone in the treatment of patients with type 2 diabetes and diabetic feet and to compare ozone with antibiotic therapy. A randomized controlled clinical trial was performed with 101 patients divided into two groups: one (n = 52) treated with ozone (local and rectal insufflation of the gas) and the other (n = 49) treated with topical and systemic antibiotics. The efficacy of the treatments was evaluated by comparing the glycemic index, the area and perimeter of the lesions and biochemical markers of oxidative stress and endothelial damage in both groups after 20 days of treatment. Ozone treatment improved glycemic control, prevented oxidative stress, normalized levels of organic peroxides, and activated superoxide dismutase. The pharmacodynamic effect of ozone in the treatment of patients with neuroinfectious diabetic foot can be anoibed to the possibility of it being a superoxide scavenger. Superoxide is considered a link between the four metabolic routes associated with diabetes pathology and its complications. Furthermore, the healing of the lesions improved, resulting in fewer amputations than in control group. There were no side effects. These results show that medical ozone treatment could be an alternative therapy in the treatment of diabetes and its complications.
q        Effect of ozone on the oral microbiota and clinical severity of primary root caries.

Baysan A , Lynch E .

The School of Dentistry, University of Birmingham, St Chad's Queensway, Birmingham B4 6NN, UK. a.baysan@bham.ac.uk

PURPOSE: To assess the effect of ozone on the microbial flora and clinical severity of primary root caries. METHODS: 26 patients with 70 primary root carious lesions (PRCLs) were entered. Each PRCL was classified in terms of color, cavitation, size, hardness, distance from the gingival margin and severity. Overlying plaque was then removed and each lesion dried. A biopsy was taken from half of each PRCL using a sterile excavator. Subsequently, the remaining lesions were exposed to ozone gas for a period of either 10 seconds (n = 35) or 20 seconds (n = 35) and a further biopsy was taken. RESULTS: Using a paired Student t-test, a significant (P < 0.001) difference (mean +/- SE) in total micro-organisms was observed in the ozone-treated samples after either a 10 seconds (log10 4.35 +/- 0.49) or 20 seconds (log10 0.46 +/- 0.26) ozone application compared with the control samples (log10 7.00 +/- 0.24) and (log10 6.00 +/- 0.21) respectively. Using Pearson's correlation tests, there were significant correlations for the reduction in total micro-organisms after 10 seconds of ozone application with cavitation, size, distance from gingival margin and severity of PRCLs (P < 0.05). In conclusion, ozone application either for 10 or 20 seconds dramatically reduced most of the micro-organisms in PRCLs without any side effects recorded at recall intervals between 3 and 5.5 months. Out of the 65 PRCLs reviewed, 33 lesions had become hard, 27 lesions reversed to severity index 1 from severity index 2, and five lesions remained the same following ozone application for a period of either 10 or 20 seconds.

Publication Types: Clinical Trial , Randomized Controlled Trial
PMID: 15241911 [PubMed - indexed for MEDLINE]